Carbohydrazide derivatives are known to be antimalarial, antitubercular, antitumor, agonist and anticancer agents. Keeping in view their potential utility, we want to synthesize novel building blocks of substituted carbohydrazide. Such building blocks can be incorporated in various biologically active molecules also in some secondary structures of proteins (beta-sheets). As these building blocks can mimic the donor acceptor behavior of naturally occurring peptides, they can potentially play significant role in the folding pattern of secondary structures of proteins. A facile synthetic route for the synthesis of this substituted carbohydrazide building blocks will be optimized and the key intermediates will be fully characterized using various analytical techniques like IR, NMR, and HRMS.