Hyperaccumulator plants could be used on polluted soil in order to remove heavy metals. Brachiaria Reptans, Cannabis sativa and Parthenium hyesterophorous are considered the most effective intaking up metals in contaminated areas of Rawalpindi and Islamabad.However, there is a prime need to identify the target genes involved in this process in order to develop transgenic plants. Therefore, in this project, we will identify the target genes involved in the heavy metal uptake. In addition, the plants produce different proteins under stress condition in order to survive and overcome stress. Among the different proteins, proteinase inhibitors “cystatins” plays an important role under biotic and abiotic stress. Our experimental system will consist of the production of recombinant cystatins in heterologous systems (such as E._coli) from medicinal plant Tagetes minuta. Production of recombinant proteins is widely used in pharmaceutical industry e.g. insulin that is used for diabetics patients, as well as in research for structural and/or biochemical studies of newly identified proteins. In this project we will identify and produce recombinant cystatins from medicinal plant Tagetes minutaand use them as biopesticides and against human diseases.