Environmental Biotechnology

Project Description

Salt stress is one of the major causes that limits agricultural productivity in world’s irrigated land. Agricultural sector faces the challenge of more food production to fulfill the needs of ever increasing population. Soil salinity creates nutrient deficiency, ion toxicity, osmotic and oxidative stress in plants. Plants have the mechanisms to adjust their internal environment to cope with adverse outer environment, e.g., by accumulating the compatible solutes like proline and glycine betaine in case of salt stress. However, such responses rely strongly on plant innate genetic potential as some plants can better perform in such stressful conditions while other plant species are unable to survive in such conditions. Unfortunately, most agricultural crops fall in the later class of plant species. Therefore, it is important to increase tolerance to salt stress of such crop plants by understanding the fundamental mechanisms and to improve the processes that naturally occur in crop plants under stressful environmental conditions like salinity.  Drought stress is another important environmental stress that limits crop productivity. Quantitative knowledge of the response of crops to water stress is vital for the better management of irrigated and dry land crops. Pea is an annual self-pollinated legume crop.  Its production is limited due to its salt and drought sensitive nature. There is need for extensive research to discover the genetic and biochemical mechanisms of pea in response to salt and drought stress. The current research is designed to investigate the underlying physiological, biochemical and molecular processes during salt stress both in hydroponics system and in soil and to find the salt stress tolerance level in three Pea varieties. In physiological and biochemical studies important ions like Na+, K+ and Ca2+ will be determined by atomic absorption spectrophotometer. Other important metabolites such as glycine betaine, jasmonates will be measured mainly by High Performance Liquid Chromatography. Enzyme assays will be performed through the standard reported protocols. In molecular part of the current research, genes like P5CS, P5CDH, GB1 etc. responsible for salt tolerance in pea crop will be identified and their phylogenetic relationship with these genes in other crops will be determined using comparative genomics approach. This study is expected to be helpful in identification of the important metabolites and genes involved in salinity response in pea crop. Also, the activities of important enzymes involved in drought response will be performed. The approaches used in this study could also be applied to other important crops to improve their capability to salt stress.

Project Funding

Sr. No.Funding BodyFunds
1 COMSATS 297000 Rs

Members

  • Dr. Sabaz Ali Khan