In this study up flow anaerobic sludge blanket (UASB) will be used to treat carwash wastewater at varying room temperature with different hydraulic retention times ranging from 4 to 36 h for three months of operational time period. The samples will be run in dilutions of 70% (30% wastewater and 70% clean water) for one week, 40% second week and then shift to 100% (without dilution of carwash wastewater). The operating conditions that will be interpreted are COD, BOD and sCOD (soluble COD), TS and VS for kinetics study. To study the hydrocarbon GC-MS (gas chromatograph and mass spectrometry) analysis will be performed before and after the treatment.  Arrhenius and monod model can be applied to calculate activation energy, substrate utilization rate and other kinetics parameters. The main objectives of this research are to study substrate utilization rate constant (k), decay rate constant (kd), yield coefficient (Yg), half velocity constant (Ks) and maximum specific growth rate (µm) of carwash wastewater treating through UASB. Kinetic modeling-based studies give a good insight into reaction mechanism and help to describe several specific parameters to monitor the system performance. This study will help us to find most economical and sustainable anaerobic system for carwash industry wastewater treatment.