Project Description
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Breast carcinoma is the second leading cause of death due to cancer among females. In Pakistan, it is more common at a young age contrary to the West where it is more common after 60 years. Many treatment options are available for this disease with major limitations of cost, effectiveness and safety. So, there is an urgent need to establish a cheap, safe and effective treatment protocol for breast cancer. Otostegia limbata (Lamiaceae) is a medicinally important plant used as folk medicine for different diseases including hypertension, inflammation, wounds and gum diseases. However, the literature lacks the scientific investigation of this plant for screening and isolation of chemical constituents for breast cancer activity. Therefore, the aim of current study is to determine the cytotoxic potential and isolation of various chemical constituents from O. limbata. For this purpose, O. limbata (leaves) will be subjected to extraction with methanol followed by bio-assay guided fractionation using solvent technique. The most active fraction(s) will be subjected to various chromatographic techniques for the isolation for various active chemical constituents. Brine-shrimp cytotoxicity and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assays will be performed for the screening of cytotoxic potential of crude extract, fractions and bioactive chemical constituents of O. limbata.
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Project Funding
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1
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COMSATS Institute of Infomation Technology
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200000
Rs
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Members
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