Abiotic stress like salinity is a major factor that significantly limits crop growth and productivity. It has been shown that various salt stress responsive genes participate in salt stress tolerance. Generation of transgenic plants with genes resistance to salt stress can greatly improve plant salinity tolerance. Plant specific kinases and transcription factors (TFs) play an important role in this regard. Genetic engineering of agriculturally important crops such as tomato for salt tolerance holds promising application. Local cultivar tomato ex-plant will be used for transformation of tomato plant with salt stress resistance gene. Once transformed with salt tolerance gene, tomato ex-plants will be put on different combination of rooting and shooting hormone concentration for tomato ex-plant growth. This will result in tomato plant growth and regeneration. The regenerated plantlets will be tested for presence of salt stress resistant gene and stress tolerance. The regenerated plantlets will be transferred to the green house to be planted in soil. This work will form the basis for transformation and development of transgenic salt stress resistance lines in local tomato variety. This study can be used for improved abiotic stress trait in tomato crop that will greatly benefit the agriculture industry of Pakistan