Rapid increase in world population demands for food security and sustainable agriculture. Among various pests, nematodes cause severe damage to world agriculture. It has been estimated that 10-12% crops have been damaged by nematode worldwide. In many cases, the symptoms of nematode infection are similar to those caused by salt stress and nutrient deficiency. This wrong interpretation results in ignoring nematode management especially in developing countries. Eco-friendly control of crop damaging nematode is necessary for sustainable agriculture. Various crops including tomato, okra, potato, beans have been reported to be damaged by nematodes. Current project will focus on isolation bacterial strains and its application to control crop damage. Isolated bacterial strains would be examined for their potential to control Caenorhabditis elegans (a model nematode) and Meloidogynae incognita (a species of most aggressive genus of phytonematodes). After initial screening, tomato seeds would be coated with the bacterial strains and grown in pots. Treated and untreated plants would be exposed to rot knot nematode and plant health would be analyzed with different growth parameters. Once, suitable bacterial strains would be isolated attempts would be made to identify genes involved in the killing of worms. This would be done creating a library of mutants and screening those mutants against worms for attenuated virulence. Successful completion of this project would result in the preparation of bacterial formulation for sustainable agriculture.