Aflatoxins are those organic compounds which are produced by the fungus on feed materials. Aflatoxin B1 is one the most hazardous aflatoxins and humans as well as poultry both are severely harmed by this toxin. It is necessary to degrade/eliminate mycotoxins from the poultry feed to ensure poultry health. The current study is designed to isolate the bacteria from the various sources which will have capability to degrade the aflatoxin B1. Isolated bacterial strain would be identified biochemically. Shake flask experiments would be carried out to determine the rate of degradation. TLC and HPLC would be the analytical method to study degradation. Co-metabolism degradation of afltatoxin B1 by newly isolated strain would be studied by supplying different sugars as extra carbon source.