Naturally occurring plants possess great biological and medicinal importance that is effective against various            diseases. Arisaema is very important, biologically active medicinal plant. The plant species is assumed to be containing significant biochemical compounds of medicinal nature. It is therefore necessary to evaluate some of the important compounds from this plant species as it has not yet been fully explored for its chemical constituents. The purpose of current study will be to isolate and characterize the flavonoids especially flavonols and anthocyanins fromArisaema found in North and Northwestern parts of Pakistan. Qualitative analysis will be performed by using the thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) techniques. Further purification and isolation will be done by using column chromatography followed by determination of antimicrobial activity of plant crude extracts and purified compounds from Arisaema. The results will provide the basic information about flavonoids present in this important plant species that will further our knowledge to identify the structure of flavonoids which will eventually be useful in drug discovery.