Most of the world’s population relies on medicinal plants for their primary health care. Plant based medicines have reached widespread acceptability as therapeutic, nutraceutics, and cosmetic agents. Medicinal plants contain variety of bioactive compounds particularly phenolic compounds that are reported to have potential antioxidant properties. Flavonoidsas natural antioxidants are abundant in plants and these play vital role in health care.It is generally observed that humans can accept naturally occurring materials more easily in comparison to synthetic compounds. There is thus a strong need for exploring effective antioxidants from natural sources to prevent deterioration of foods. Pakistan has great potential for drug discovery through the use of natural products abundantly present in medicinal plant flora.  Due to its geographical and climatic conditions Pakistan captures a unique position of having a wide diversity of medicinal plants growing over a range from cool temperate to humid and hot tropics.  These varied edaphic and climatic factors of the country reflect diversity and valuable medicinal heritage. Five medicinal plants species including four species of Roscoea i.e., R. capitata, R. auriculata,R. alpine,R. purpurea and one species of Dicliptera D. bupleuroides would be selected from Northern regions (Hazara Div, Margalla and lesser Hamalaya) for isolation and characterization  of flavonoids (flavonols, anthocyanidins, flavones, flavanones, catechins and isoflavones). These plants hold significant medicinal importance and are rich in bioactive components. The proposed project will analyze total flavonoids in plant extracts. The antioxidant activity of extracts and isolates will be evaluated by radical scavenging assays. By the completion of the proposed project, these natural resources will be conserved with derived products useful for health.  Additional benefits would be to support pharma, food and biotech industries through potential products and processes.